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KMID : 0380620130450020156
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Korean Journal of Food Science and Technology
2013 Volume.45 No. 2 p.156 ~ p.160
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Development of Detection Method of Unapproved Genetically Modified Potato (EH92-527-1) in Korea using Duplex Polymerase Chain Reaction
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Yoo Myung-Ryul
Kim Jae-Hwan
Kim Hea-Yeong
Yea Mi-Chi
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Abstract
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A duplex polymerase chain reaction (PCR) method was developed to detect unapproved genetically modified (GM) potato (EH92-527-1) in Korea. The UDP-glucose pyrophosphorylase (UGP) gene was selected as an endogenous reference gene for potato and used to validate the specificity for 14 different crops. The primer pair EH92-F/R was designed to amplify the junction sequence between the genome and transgenic region introduced in GM potato. Its specificity was also validated using several different GM events. The detection limit of the duplex PCR method is approximately 0.05%. This duplex PCR method could be useful for monitoring cultivation of unauthorized GM potato in Korea.
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KEYWORD
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EH92-527-1, genetically modified potato, duplex PCR, UDP-glucose pyrophosphorylase
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